Tugboat Annie Sails Again was a 1940 sequel to the classic 1933 film Tugboat Annie. Marjorie Rambeau takes over the late Marie Dressler's role, and the supporting cast includes Alan Hale, Jane Wyman, and Ronald Reagan. The movie was directed by Lewis Seiler. Another sequel appeared five years later called Captain Tugboat Annie with Jane Darwell taking over the title role.
* Marjorie Rambeau as Annie Brennan
* Alan Hale, Sr. as Horatio Bullwinkle
* Jane Wyman as Peggy Armstrong
* Ronald Reagan as Eddie Kent
* Chill Wills as Shiftless