Later that month, the King was unhorsed in a tournament and knocked unconscious for two hours, a worrying incident that Anne believed led to her miscarriage five days later. On the day that Catherine of Aragon was buried at Peterborough Abbey, Anne miscarried a baby which, according to the imperial ambassador Chapuys, she had borne for about three and a half months, and which "seemed to be a male child". For Chapuys, this personal loss was the beginning of the end of the royal marriage.
Given Henry's desperate desire for a son, the sequence of Anne's pregnancies has attracted much interest. Author Mike Ashley speculated that Anne had two stillborn children after Elizabeth's birth and before the birth of the male child she miscarried in 1536. Most sources attest only to the birth of Elizabeth in September 1533, a possible miscarriage in the summer of 1534, and the miscarriage of a male child, of almost four months gestation, in January 1536.